Cryo-EM✗ Internal Only

Cryo-Electron Microscopy Facility (Janelia Research Campus)

Howard Hughes Medical Institute - Janelia Research Campus

📍 Ashburn, VA · Website ↗

Janelia's Cryo-Electron Microscopy Facility, directed by Zhiheng Yu, is an HHMI-wide shared facility that supports the full workflow from grid preparation through data collection and image processing. It operates four Titan Krios microscopes (Krios 1 with Cs corrector, BioContinuum HD and K3; Krios 2 with BioQuantum and K3, scheduled for retirement in May 2026 and replacement by a Krios 5; Krios 3 and Krios 4 with cold FEG, Selectris X and Falcon 4i), a Talos L120C and Tecnai F20 for screening, an Aquilos 2 and a Hydra plasma cryo-FIB-SEM, two Vitrobot Mark IVs, Leica EM GP and GP2 plungers, a chameleon, and Leica cryo-CLEM widefield and Stellaris 8 cryo-confocal microscopes.

Cryo-Electron Microscopy Facility (Janelia Research Campus) serves its own institution only. These can take outside samples:

Virginia facilities that take external users

Private providers that run samples

Contact providers directly. We don't broker quotes or pass on your details.

Equipment Inventory(11 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Hydra Bio

Thermo Fisher Scientific

FIB-SEM
Configuration: Cryo plasma FIB-SEM (Xe, Ar, O2, N2 ion sources); 100-300 nm lamellae; iFLM; cryo-SEM imaging; page name is 'FEI Hydra Cryo Plasma FIB-SEM'

Aquilos 2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM
Configuration: Gallium cryo-FIB-SEM; 100-300 nm lamellae; integrated fluorescence light microscopy (iFLM)

Leica Stellaris 8 cryo-confocal

Leica

Other
Configuration: Confocal with cryo stage; FLIM capable

Leica EM Cryo CLEM

Leica Microsystems · Cryo-CLEM

Other
Configuration: Wide-field fluorescence imaging of cryo grids for CLEM

Vitrobot Mark IV

×2

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: Two plunge freezers

Leica EM GP

Leica

Sample Prep
Configuration: Cryo plunger

Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep
Configuration: Cryo plunger

chameleon

SPT Labtech

Sample Prep
Configuration: Blot-free nanoliter dispensing onto self-wicking grids

Titan Krios

×4

Thermo Fisher Scientific

TEM
Configuration: Four instruments. Krios 1, installed 2011 and upgraded since, X-FEG with Cs corrector, Gatan BioContinuum HD energy filter, post-filter K3, Ceta, STEM. Krios 2, installed 2013, X-FEG, BioQuantum energy filter, post-filter K3, OneView, scheduled for replacement by a Krios 5 in the same room. Krios 3, operational September 2021, cold FEG, Selectris X, Falcon 4i, Ceta, STEM. Krios 4, installed 2025, cold FEG, Selectris X, Falcon 4i, Ceta.

Talos L120C

Thermo Fisher Scientific · Screening / Negative Stain

TEM
Voltage: 120 kV
Configuration: 120 kV screening TEM; camera not stated

Tecnai F20

Thermo Fisher Scientific

TEM
Configuration: 200 kV screening TEM; camera not stated