Cryo-EM✓ External Access

UW-Madison Cryo-Electron Microscopy Research Center (CEMRC)

University of Wisconsin-Madison

📍 Madison, WI · Website ↗

The Cryo-Electron Microscopy Research Center (CEMRC) in the Hector F. DeLuca Biochemical Sciences Complex operates a 300 kV Titan Krios G3i, a 200 kV Talos Arctica and a 120 kV Talos L120C plus Aquilos 2 cryo-FIB-SEM and plunge-freezing equipment for single particle analysis, cryo-electron tomography and MicroED. It also hosts the Midwest Center for Cryo-Electron Tomography (MCCET, NIH U24 GM139168), which adds a cold-FEG Titan Krios G4i with Selectris X energy filter and Falcon 4i, an Aquilos 2 with integrated fluorescence microscope and a Leica Stellaris 5 cryo-confocal. Rates are posted for internal, external academic and industry users, and MCCET time is allocated through the National Cryo-ET Network hub at cryoetportal.org.

Equipment Inventory(10 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Aquilos 2

×2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM
Configuration: Two separate cryo-FIB-SEM instruments: one MCCET unit with integrated fluorescence light microscope (iFLM) for correlative lamella prep, one CEMRC unit; UW equipment page lists it generically as Aquilos cryo-FIB-SEM

Leica Stellaris 5 Cryo-Confocal

Leica Microsystems

Other
Configuration: Cryo-confocal light microscope for correlative cryo-ET workflows, MCCET

Vitrobot Mark IV

×2

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: Plunge freezers, one at each of the centre's two programmes, bookable through iLab.

Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep
Configuration: Automatic plunge freezer, MCCET

Leica EM ACE600

Leica Microsystems · Coater

Sample Prep
Configuration: High vacuum sputter/carbon coater for grid preparation

GloQube

Quorum · Glow Discharge

Sample Prep
Configuration: Glow discharge system for grid hydrophilization. An easiGlow unit is also available.

Titan Krios G4i

Thermo Fisher Scientific

TEM
Configuration: 300 kV, cold FEG, Volta phase plates, Panther STEM detectors, Falcon 4i, Selectris X energy filter with Falcon 4i, Ceta-D CMOS; MCCET instrument funded by NIH U24 GM139168

Talos L120C

Thermo Fisher Scientific · Screening / Negative Stain

TEM
Voltage: 120 kV
Configuration: 120 kV, LaB6 source, Ceta CMOS; negative stain screening and cryo-TEM; CEMRC-owned

Talos Arctica

Thermo Fisher Scientific · Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: 200 kV, Schottky FEG, Volta phase plates, Falcon 3C, Gatan BioQuantum energy filter with K3, Ceta CMOS; used for cryo screening and initial data collection; CEMRC-owned

Titan Krios G3i

Thermo Fisher Scientific · Single Particle / Tomography

TEM
Voltage: 300 kV
Configuration: 300 kV, Schottky FEG, Volta phase plates, STEM detectors, Falcon 3C, Gatan BioQuantum energy filter with K3, Ceta-D CMOS; fringe-free imaging and MicroED hardware added in 2022 upgrade; CEMRC-owned

Rates

This facility publishes its rates as a document rather than a web page. View the facility's rate document →