Cryo-EM✓ External Access

Electron Microscopy Shared Resource (EMSR)

Fred Hutchinson Cancer Center

📍 Seattle, WA · Website ↗

The Electron Microscopy Shared Resource at Fred Hutch, directed by Theo Humphreys, provides negative-stain and cryo-EM grid preparation, screening, data collection and processing, plus conventional TEM and SEM sample processing. It operates a 200 kV Glacios with an X-FEG and Gatan K3 detector, a 120 kV Talos L120C with a Ceta-M camera and Elsa cryo holder, a JEOL JSM-6610LV SEM, a Vitrobot Mark IV and a Leica EM ICE high-pressure freezer. Users can also reach the UW Beckman Center Titan Krios and PNCC for higher-end data collection.

Equipment Inventory(6 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: Plunge freezer; Pelco easiGlow glow discharger, Denton benchtop turbo coater and Cressington carbon coater alongside

Leica EM ICE

Leica

Sample Prep
Configuration: High-pressure freezer with Leica AFS2 automatic freeze substitution

Leica EM UC7

Leica Microsystems · Ultramicrotome

Sample Prep
Configuration: Ultramicrotome(s) for ultrathin and semithin sectioning (page uses plural); Leica VT1200S vibratome also available

JEOL JSM-6610LV

JEOL

SEM
Configuration: 0.3-30 kV; low-vacuum SEM; Tousimis critical point dryer and Denton Desk IV sputter coater for prep

Glacios

Thermo Fisher Scientific · Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: 200 kV X-FEG; Gatan K3 direct detector (24 MP, 1500 fps); 12-grid autoloader; screening and data collection

Talos L120C

Thermo Fisher Scientific · Screening / Negative Stain

TEM
Voltage: 120 kV
Configuration: 120 kV LaB6; Ceta-M 16M CMOS camera; single-tilt room-temperature holder and Elsa cryo-transfer holder; TIA, Velox, Leginon

Rates

This facility publishes its rates as a document rather than a web page. View the facility's rate document →