Cryo-EM✓ External Access

CSB Cryo-Electron Microscopy Facility

Vanderbilt University

📍 Nashville, TN · Website ↗

The CSB Cryo-Electron Microscopy Facility is run by Vanderbilt's Center for Structural Biology, with microscopes in the Engineering and Science Building and a sample preparation room in Medical Center North. It operates a 300 kV Titan Krios G4 with Gatan K3 BioQuantum, Falcon 3 and Volta phase plate (operational April 2021), a 200 kV Glacios with Falcon 4i for screening, SPA, tomography and MicroED, and a Talos L120C with Ceta-S camera for negative stain. Sample preparation uses Vitrobot Mark II and Mark IV plungers and a Leica EM GP2, and data are processed on GPU workstations with 600 TB of local storage.

Equipment Inventory(6 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: Medical Center North RR1207

Vitrobot Mark II

FEI

Sample Prep
Configuration: Medical Center North RR1207

Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep
Configuration: ESB room 050

Titan Krios G4

Thermo Fisher Scientific · Single Particle / Tomography

TEM
Voltage: 300 kV
Configuration: 300 kV; Gatan K3 with BioQuantum energy filter; Falcon 3; Volta phase plate; ESB room 003; fully operational April 2021

Glacios

Thermo Fisher Scientific · Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: 200 kV; Falcon 4i; ESB room 050; screening, SPA, tomography, MicroED

Talos L120C

Thermo Fisher Scientific · Screening / Negative Stain

TEM
Voltage: 120 kV
Configuration: Listed as 'L120C 100kV TEM'; Ceta-S 4k x 4k CMOS; ESB room 504; negative stain screening