Cryo-EM✓ External Access

Pittsburgh Center for CryoEM (PCCEM)

University of Pittsburgh at Pittsburgh

📍 Pittsburgh, PA · Website ↗

The Pittsburgh Center for CryoEM is run by the Department of Structural Biology in the basement of Biomedical Science Tower 3. It operates a Titan Krios G3i with Selectris energy filter, Volta phase plate and Falcon 4i, a Tecnai TF20 with TVIPS XF416 camera for screening, a Tecnai T12 for negative stain, and an Aquilos 2 cryo-FIB, with a Vitrobot Mark IV for plunge freezing. Access is offered in Independent, Assisted and Full service tiers with separate academic and external pricing.

Equipment Inventory(7 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Aquilos 2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM

Configuration: Cryo-FIB for lamella milling of cellular samples

Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep

Configuration: Plunge freezer

Emitech K100X

Quorum · Glow Discharge

Sample Prep

Configuration: Glow discharger

Fischione 1020

Fischione

Sample Prep

Configuration: Plasma cleaner

Tecnai F20

Thermo Fisher Scientific

TEM

Configuration: 200 kV TF20; TVIPS XF416 CMOS camera; Gatan 910 cryoholder (three grids); SerialEM; used for cryo-grid screening and data collection

Tecnai T12

Thermo Fisher Scientific

TEM

Configuration: 120 kV; LaB6 source; Gatan Orius CCD; negative stain

Titan Krios G3i

Thermo Fisher Scientific · Single Particle / Tomography

TEM

Voltage: 300 kV

Configuration: 300 kV; Selectris energy filter; Volta phase plate; Falcon 4i; facility writes it as 'Krios 3Gi'

Rates

This facility publishes its rates as a document rather than a web page. View the facility's rate document →