Cryo-EMโœ“ External Access

Beckman Center for Cryo-Electron Microscopy

University of Pennsylvania

๐Ÿ“ Philadelphia, PA ยท Website โ†—

The Beckman Center for Cryo-Electron Microscopy is part of the Perelman School of Medicine's Electron Microscopy Resource Laboratory and is housed in room G011 of the Singh Center for Nanotechnology, with cryo-ET sample preparation space in the Anatomy Chemistry Building. It operates a 300 keV Titan Krios G3i with K3, BioQuantum filter and Volta phase plate, and a 200 keV Glacios 2 with Falcon 4i and Selectris X. The associated Beckman Center for Cryo-Electron Tomography adds a Zeiss Crossbeam 550 cryo-FIB-SEM with Delmic Meteor, a Zeiss LSM 900 cryo-confocal, Alveole micropatterning, and Vitrobot Mark IV and Leica EM GP plungers.

Equipment Inventory(7 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Zeiss Crossbeam 550

Zeiss

FIB-SEM
Configuration: Linkam cryostage; Delmic Meteor in-chamber fluorescence microscope; used for cryo-lamella prep and volume SEM

Zeiss LSM 900

Zeiss

Other
Configuration: Laser scanning confocal with cryostage for correlative workflows

Leica EM GP

Leica

Sample Prep
Configuration: Plunge freezer (GP vs GP2 not stated)

Vitrobot Mark IV

Thermo Fisher Scientific ยท Vitrification

Sample Prep
Configuration: Plunge freezer

Alveole PRIMO micropatterning

Alveole

Sample Prep
Configuration: Micropatterning of EM grids for cell growth

Glacios 2

Thermo Fisher Scientific ยท Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: 200 kV; Autoloader; Falcon 4i DED; Selectris X energy filter; fringe-free imaging; EPU Multigrid

Titan Krios G3i

Thermo Fisher Scientific ยท Single Particle / Tomography

TEM
Voltage: 300 kV
Configuration: 300 kV; Autoloader; Gatan K3 DED; BioQuantum K3 imaging filter; Volta phase plate; fringe-free imaging; EPU Multigrid
Internal: $60/hr

Published Rates

As published by the facility, retrieved Aug 10, 2026 ยท source. Rates change; confirm with the facility before budgeting.

ServiceRateApplies toNotes
Electron cryo-microscopy (hourly)$120/hrโ€”โ€”
Plunge freezing$125/sampleโ€”โ€”
Single particle data collection (hourly)$100/hrโ€”โ€”
T12 / JEOL 1010 (hourly)$50/hrโ€”โ€”
TF-20 EPU run (24 hr)$1500/24hrโ€”โ€”
TF-20 use (assisted)$125/hrโ€”โ€”
Tomography image construction$450/tomoโ€”dual axis

Pricing

InstrumentInternal RateExternal RateNotes
Titan Krios G3i$60/hrโ€”Instrument use approx $40-$60/hr. Staff time $100-$163/hr depending on service level.