Cryo-EM✓ External Access

Simons Electron Microscopy Center (SEMC)

New York Structural Biology Center

📍 New York, NY · Website ↗

The Simons Electron Microscopy Center at NYSBC (89 Convent Ave, New York) runs three access programs: the Members Electron Microscopy Center for member institutions, and the NIH national centers NCCAT (single-particle access and training) and NCITU (in situ tomography). MEMC lists three named Titan Krios (Jim, Marilyn, Audrey), a Glacios and a Hitachi HT7800; NCCAT lists six Titan Krios (including one cold-FEG G4-class instrument), two Glacios and a Hitachi 7800; NCITU adds an Aquilos 2 cryo-FIB-SEM and Zeiss LSM 900 cryo-CLEM. Sample preparation includes Vitrobot Mark IV, Leica EM GP and GP2, SPT Labtech chameleon and Refeyn mass photometry.

Equipment Inventory(12 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Aquilos 2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM
Configuration: NCITU cryo-FIB-SEM for lamella thinning

Zeiss LSM 900

Zeiss

Other
Configuration: confocal cryo-CLEM with Airyscan 2 detector and Linkam cryostage; listed by both NCITU and MEMC

Refeyn One MP

Refeyn

Other
Configuration: mass photometer

Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep
Configuration: NCCAT plunge freezer

Leica EM GP

Leica

Sample Prep
Configuration: MEMC plunge freezer

chameleon

SPT Labtech

Sample Prep
Configuration: Blot-free vitrification robot, available through both the member centre and the national centre.

Gatan Solarus

×2

Gatan · Plasma Cleaner

Sample Prep
Configuration: Solarus and Solarus II plasma cleaners / glow dischargers

Leica EM UC6

Leica Microsystems · Ultramicrotome

Sample Prep
Configuration: ultramicrotome at MEMC

Vitrobot Mark IV

×2

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: one listed by MEMC, one listed by NCCAT ('FEI Vitrobot IV')

Titan Krios

×9

Thermo Fisher Scientific

TEM
Configuration: MEMC Krios #1 'Jim'; 300 kV; Cs 2.7 mm; Volta Phase Plate; Autoloader; Gatan K3, BioQuantum imaging filter, Ceta CMOS; MEMC Krios #2 'Marilyn'; 300 kV; Cs image corrector; Volta Phase Plate; Autoloader; Gatan K3, BioQuantum imaging filter, Ceta CMOS; MEMC Krios #3 'Audrey'; 300 kV; Cs 2.7 mm; Volta Phase Plate; Autoloader; Falcon III, Gatan K3, Ceta CMOS; NCCAT; 300 kV; Falcon 4i direct detector; energy filter; Cs 2.7 mm; three separate entries on the NCCAT instrumentation page; NCCAT; 300 kV; Gatan K3 direct detector; energy filter; Cs 2.7 mm; NCCAT; 300 kV; Gatan K3 direct detector; energy filter; Volta Phase Plates; Cs 2.7 mm | NCCAT cold-FEG unit: NCCAT; 300 kV cold FEG (cFEG); Falcon 4i direct detector; Cs 2.7 mm

HT7800

Hitachi

TEM
Configuration: MEMC 'Marcia'; 100 kV; room-temperature side-entry holder; TVIPS F416 CMOS; NCCAT lists a 'Hitachi 7800 120kV' with TVIPS F416 that may be the same instrument

Glacios

×3

Thermo Fisher Scientific · Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: MEMC Glacios #2 'Walter'; 200 kV; Autoloader; Falcon III, Ceta CMOS; NCCAT; 200 kV; Ceta-D CMOS and Falcon III direct detector; NCCAT; 200 kV; Ceta-D CMOS and Falcon IV direct detector