Cryo-EM✓ External Access

Washington University Center for Cellular Imaging (WUCCI)

Washington University

📍 Saint Louis, MO · Website ↗

WUCCI is the core imaging facility of the Washington University School of Medicine in St. Louis, covering light, electron, X-ray and cryo-electron microscopy. Its cryo-EM unit runs a 300 kV Titan Krios G3 with Falcon 4i and Selectris X energy filter, a 200 kV Glacios with Falcon 4 and Gatan K3 cameras, and an Aquilos 2 cryo-FIB, supported by a Vitrobot Mark IV, a Leica EM ICE high-pressure freezer and a Leica sample preparation suite. Rates are posted for WashU, external academic and commercial users.

Equipment Inventory(14 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Aquilos 2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM
Configuration: Cryo-FIB with NICol UHR SEM column (Schottky FEG) and Sidewinder gallium ion source; cell and tissue lamella preparation

Leica EM AFS2

Leica Microsystems · Freeze Substitution

Sample Prep
Configuration: Automated freeze substitution

Leica EM ACE600

Leica Microsystems · Coater

Sample Prep
Configuration: Sputter coater and carbon thread evaporator

Leica EM ACE900

Leica Microsystems · Coater

Sample Prep
Configuration: Freeze fracture system for platinum replicas

Leica EM CPD300

Leica Microsystems · Critical Point Dryer

Sample Prep
Configuration: Critical point drier

Gatan Solarus 950

Gatan

Sample Prep
Configuration: Plasma cleaner

GloQube

Quorum · Glow Discharge

Sample Prep
Configuration: Glow discharger with clean and vapor chambers

Leica EM UC7

×2

Leica Microsystems · Ultramicrotome

Sample Prep
Configuration: Two ultramicrotomes; one FC7 cryo stage attachment

Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep
Configuration: Listed as Vitrobot Mk IV

Leica EM ICE

Leica

Sample Prep
Configuration: High-pressure freezer with light stimulation

Zeiss Merlin

Zeiss

SEM
Configuration: FE-SEM with GEMINI II column, ATLAS 5.0 scan engine, in-lens and solid-state STEM detectors

Glacios

Thermo Fisher Scientific · Single Particle / Screening

TEM
Voltage: 200 kV
Configuration: 200 kV; Falcon IV direct electron detector; Gatan K3 direct electron detector; Ceta-D camera for MicroED; phase plate; STEM detector; autoloader

JEOL JEM-1400

JEOL · Screening / Negative Stain

TEM
Voltage: 120 kV
Configuration: 120 kV room-temperature TEM; AMT XR111 4k x 2k CCD

Titan Krios G3

Thermo Fisher Scientific · Single Particle / Tomography

TEM
Configuration: 300 kV; Falcon 4i direct electron detector with Selectris X energy filter; Ceta 16M CMOS camera; image corrector; phase plate; STEM detector; autoloader

Published Rates

As published by the facility, retrieved Aug 10, 2026 · source. Rates change; confirm with the facility before budgeting.

ServiceRateApplies toNotes
Krios cryo-EM (per hour, assisted)$183externalacademic
Krios cryo-EM (per hour, assisted)$381industrycommercial
Krios cryo-EM (per day, trained users)$1,940internalWashU
Krios cryo-EM (per hour, assisted)$122internalWashU peak; FY from Jul 1 2026