Cryo-EM✓ External Access

Southeastern Center for Microscopy of MacroMolecular Machines (SECM4)

Florida State University

📍 Tallahassee, FL · Website ↗

SECM4 is an NIH R24 GM145964 funded service center co-located with the Biological Sciences Imaging Resource at Florida State University. It provides cryo-EM specimen preparation, grid screening, high-resolution data collection, and training to biomolecular researchers primarily in the Southeast. Instruments are a 300 kV Titan Krios with a Direct Electron Apollo camera, Gatan BioQuantum K3, and Volta phase plate, a Hitachi HT7800 120 kV screening TEM, a Vitrobot, a Leica EM GP2, and an SPT Labtech chameleon.

Equipment Inventory(5 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Vitrobot

Thermo Fisher Scientific

Sample Prep
Configuration: Plunge freezer for grid vitrification.

Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep
Configuration: Automatic plunge freezer, sensor-controlled blotting

chameleon

SPT Labtech

Sample Prep
Configuration: Blot-free pico-litre vitrification

Titan Krios

Thermo Fisher Scientific

TEM
Configuration: 300 kV, third-generation autoloader, Direct Electron Apollo camera, Gatan BioQuantum K3 energy filter/camera, FEI Volta phase plate, magnetic field compensation; 1.6 A apoferritin

HT7800

Hitachi

TEM
Configuration: 20-120 kV RuliTEM, TVIPS 2k x 2k camera, +/-70 degree tilt, W or LaB6 gun; grid screening