Cryo-EM✓ External Access

Center for Structural Biology Cryo-EM Facility

Vanderbilt University

📍 Nashville, TN · Website ↗

Two-site cryo-EM facility (Engineering Science Bldg and Medical Center North) supporting SPA, tomography, and MicroED for the Vanderbilt research community.

Equipment Inventory(5 instruments)

Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep

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Recommended Consumables

for Vitrobot Mark IV

Whatman #1 Blotting Paper

Ted Pella · via Amazon

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Ethane Gas (Research Grade)

Electron Microscopy Sciences · via Direct

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Vitrobot Filter Paper

Thermo Fisher Scientific · via Direct

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Leica EM GP2

Leica Microsystems · Vitrification

Sample Prep

Titan Krios G4

Thermo Fisher Scientific · Single Particle / Tomography

TEM

Voltage: 300 kV

Detector: Falcon 4i

Filter: Selectris X

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Recommended Consumables

for Titan Krios G4

R1.2/1.3 Cu 300 Mesh Grids

Quantifoil · via Direct

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C-Flat 1.2/1.3 Holey Carbon Grids

Electron Microscopy Sciences · via Direct

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Compatible Accessories

for Titan Krios G4

AutoGrid Assembly Kit

Thermo Fisher Scientific · via Direct

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Standard Software Protocols

for Titan Krios G4

cryoSPARC License (Academic)

Structura Biotechnology · via Direct

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Glacios

Thermo Fisher Scientific · Single Particle / Screening

TEM

Voltage: 200 kV

Detector: Falcon 4

Talos L120C

Thermo Fisher Scientific · Screening / Negative Stain

TEM

Voltage: 120 kV

Detector: Ceta-D