Cryo-EM✓ External Access

Pittsburgh Center for CryoEM (PCCEM)

University of Pittsburgh at Pittsburgh

📍 PITTSBURGH, PA · Website ↗

Core facility in BST3 dedicated to high-resolution structural analysis of proteins, viruses, macromolecular assemblies, organelles, and bacterial cells. Offers Independent, Assisted, and Full service tiers.

Equipment Inventory(5 instruments)

Aquilos 2

Thermo Fisher Scientific · Cryo-FIB Milling

FIB-SEM

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Recommended Consumables

for Aquilos 2

AutoGrid Cryo-FIB Clipping Rings

Thermo Fisher Scientific · via Direct

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Compatible Accessories

for Aquilos 2

Cryo-FIB AutoGrid Assembly Tool

Thermo Fisher Scientific · via Direct

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Vitrobot Mark IV

Thermo Fisher Scientific · Vitrification

Sample Prep

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Recommended Consumables

for Vitrobot Mark IV

Whatman #1 Blotting Paper

Ted Pella · via Amazon

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Ethane Gas (Research Grade)

Electron Microscopy Sciences · via Direct

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Vitrobot Filter Paper

Thermo Fisher Scientific · via Direct

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Titan Krios G3i

Thermo Fisher Scientific · Single Particle / Tomography

TEM

Voltage: 300 kV

Detector: K3

Filter: BioQuantum

Tecnai F20

Thermo Fisher Scientific

TEM

Tecnai T12

Thermo Fisher Scientific

TEM