Cryo-EM✓ External Access

BioChemistry Krios Electron Microscopy Facility (BioKEM)

University of Colorado Boulder

📍 Boulder, CO · Website ↗

BioKEM is the Biochemistry Department Krios Electron Microscopy Facility at CU Boulder, completed in June 2020 and fully operational in August 2020. It operates a 300 kV Titan Krios G3i with Falcon 4i and Selectris and a 200 kV Glacios with a Gatan K3 for single particle analysis, cryo-ET, and MicroED. Sample vitrification is referred to Boulder EM Services (BEMS) in the MCDB department, and the facility also serves as the imaging arm of the NIH-funded CU Boulder Center for Cryo-ET (CCET, U24GM139174), which is a separate program with its own site at ccet.colorado.edu.

Equipment Inventory(2 instruments)

Roster verified against the facility's own published instrument list, September 2026.

Titan Krios G3i

Thermo Fisher Scientific · Single Particle / Tomography

TEM

Voltage: 300 kV

Configuration: 300 kV (80-300 kV), X-FEG, Falcon 4i DED, Selectris imaging filter, Ceta 24M CMOS, Volta phase plate, cryo-autoloader (12 autogrids); HHMI funded; online 2020; CCET facilities page lists it as 'TITAN KRIOS F4 Selectris'

Glacios

Thermo Fisher Scientific · Single Particle / Screening

TEM

Voltage: 200 kV

Configuration: 200 kV (40-200 kV), X-FEG, Gatan K3 DED, Ceta 16M CMOS, cryo-autoloader (12 autogrids), EPU, EPU-D (MicroED), SerialEM; online December 2024; funded by Pfizer, Caruthers, Cech gifts

Rates

This facility publishes its rates as a document rather than a web page. View the facility's rate document →